Adaptation to Chlorinated Solvents Environmental Microbial Genomics Group

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Laboratoire Ampere
Ecole Centrale de Lyon . France
(33) 472 18 65 14




Origin of Dehalogenases and De Novo Dehalogenase Gene Synthesis

While our research twenty years ago dealt with the biodegradation of chlorinated compounds, the capability of bacteria to degrade these compounds is no longer in question.  Our work has evolved towards trying to understand where and how this degradative capacity arises.  Several model compounds have been used such as lindane, which is the gamma isomer of hexachlorocyclohexane, and tetra- and tri-chloroethylene (PCE and TCE), polychlorinated biphenyls (PCBs) and now Chlordecone.  Today, we are exploring the genetic resources available and necessary for the adaptation (e.g., the development of a metabolic pathway) to chlorinated compounds.  Part of this work is related to analyzing microbial communities stressed by these compounds and part is through the laboratory synthesis of the appropriate genes from non-polluted metagenomic DNA. In addition, metagenomic, metatranscriptomic and metaproteomic approaches are being applied to understand the importance of teh microbial community on dechloriantion. This basic knowledge is also applied to the clean-up of polluted sites and a video gives a hint about how. The application of metagenomics to the dechlorination of PCE was integrated into a visual 3D model of the relative annotated sequences. The MacIntosh enabled program can be downloaded as a zip file:

Evasol Axelera Biodechlor


Nemir, A., David, M.M., Perrussel, R., Sapkota, A., Pascal Simonet, P., Jean-Michel Monier, J.M., Vogel, T.M. 2010. Comparative phylogenetic microarray analysis of microbial communities in TCE-contaminated soils. Chemosphere, 80: 600-607.

Lyon, D. Y., Pivetal, J., Blanchard, L. and Vogel, T.M. 2010. Bioremediation via
In Situ Electrotransformation
, Bioremediation Journal, 14:109-119

Boubakri, H, Beuf, M., Simonet, P., Vogel, T.M., 2006. Development of metagenomic DNA shuffling for the construction of a xenobiotic gene, Gene, 375: 87-94.

Cérémonie, H., Boubakri, H., Mavingui, P., Simonet, P. Vogel, T.M. 2006. Plasmid-Encoded Gamma-Hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+)  FEMS Microbiol. Lett. 257:243-252.

Boubakri, H., H. Ceremonie, D. Bernillon, P. Simonet and T. M. Vogel. 2005. Compréhension de la structure mosaïque d'un gène: linA. Les actes du BRG 5:497-509.

Nalin, R., Simonet, P., Vogel, T.M., and Normand, P. 1999. Rhodanobacter lindaniclasticus gen. Nov., sp. Nov., a lindane-degrading bacteriumInternational Journal of Systematic Bacteriology , 49 :19-23.

Thomas, J.-C., Berger, F., Jacquier, M., Bernillon, D., Baud-Grasset, F. Truffaut, N., Normand, P., Vogel, T.M., and Simonet, P. 1996. Isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium. J. Bacteriology, 178:6049-6055.

Petrovskis, E.A., Vogel, T.M., and Adriaens, P. 1994.  Effects of electron donors and acceptors on carbon tetrachloride transformation by Shewanella putrefaciens. FEMS Microbiology Letters. 121:357-364.

Montgomery, L. and Vogel, T.M.  1992. Dechlorination of 2,3,5,6-tetrachlorobiphenyl by a phototrophic enrichment culture. FEMS Microbiology Letters , 94:247-250.

Nies, L. and Vogel, T.M.  1991.  Identification of the proton source for the microbial reductive dechlorination of a chlorinated aromatic compound (2,3,4,5,6 penta chlorobiphenyl PCB)Appl. Environ. Microbiol. 57:2771-2774.

Assaf-Anid, N., Nies, L., and Vogel, T.M.  1991.  Reductive dechlorination of a PCB congener and hexachlorobenzene by vitamin B12. Appl. Environ. Microbiol.. 58:1057-1060.

Vogel, T.M., Criddle, C.S. and McCarty, P.L. 1987.  Transformations of halogenated aliphatic compoundsEnvironmental Science and Technology, 21:722-736.

Vogel, T.M. and McCarty, P.L. 1987.  Abiotic and biotic transformations of 1,1,1-trichloroethane under methanogenic conditionsEnvironmental Science and Technology, 21:1208-1213.

Vogel, T.M. and McCarty, P.L. 1985.  Biotransformation of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under methanogenic conditionsAppl. Environ. Microbiol. , 49:1080-1083.