Fate of Transgenes from GMOs Environmental Microbial Genomics Group

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Laboratoire Ampere
Ecole Centrale de Lyon . France
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Fate of Transgenes from Transgenic Plants

Transgenes in transgenic plants can potentially reach a wide range of environmental micro-organisms. We have shown that :

1) pathogens can develop in transgenic plants and release plant DNA.
2) soil bacteria can co-infect plants undergoing necrosis.
3) gene transfer between the plant pathogen and a model soil bacteria can occur in planta.
4) for measurable gene transfer, sequences homologous to plant DNA are required.
5) plant DNA is drastically degraded before leaving the plant.
6) plant DNA making it to the soil is also dramatically reduced.

septante transbac ploben


Demanèche S, Monier J-M, Dugat-Bony E and Simonet P. 2011. Exploration of horizontal gene transfer between transplastomic tobacco and plant‐associated bacteria. FEMS Microb. Ecol. 78: 129-136.

Poté J, Ceccherini MT, Rosselli W, Wildi W, Simonet P and Vogel T.M. 2010. Leaching and transformability of transgenic DNA in unsaturated soil columns. Ecotoxicology and Environmental Safety. 73:67-72.

Pontiroli A, Ceccherini MT, Poté J, Wildi W, Kay E, Nannipieri P, Vogel T.M., Simonet P, Monier JM. 2010. Long-term persistence and bacterial transformation potential of transplastomic plant DNA in soil. Res Microbiol. 161:326-34.

Poté, J., Mavingui, P., Navarro, E., Rosselli, W., Wildi, W., Simonet, P., M. Vogel, T.M. 2009. Extracellular Plant DNA in Geneva Groundwater and Traditional Artesian Drinking Water Fountains. Chemosphere.

Demanèche S, Sanguin H, Poté J, Navarro E, Bernillon D, Mavingui P,  Wildi W, Vogel TM and Simonet P. 2008 Antibiotic resistant soil  bacteria in transgenic plant fields. (Proc Natl Acad. Sci. USA) PNAS. 105:3957-3962

Mercier, A., Bertolla, F., Passelègue-Robe E. and P. Simonet. 2007.  Natural transformation-based foreign DNA acquisition in a Ralstonia  solanacearum mutS mutant. Res. Microbiol. 158: 537-544.

Ceccherini, M.T., Ascher, J., Pietramellara, G., Vogel, T.M. and Nannipieri, P. 2007. Vertical advection of extracellular DNA by water capillarity in soil columns Soil Biology and Biochemistry, 39:158-163

Mercier, A., E. Kay, T. M. Vogel and P. Simonet. 2007. Gene flow in  the rhizosphere. In Pinton, R., Z. Varaniniand P. Nannipieri (ed.),  The Rhizosphere, Biochemistry and Organic Substances at the Soil-Plant  Interface. CRC Press, Boca Raton, FL.

Pontiroli A, Simonet P, Frostegard A, Vogel TM and JM Monier. 2007.  Fate of transgenic plant DNA in the environment. Environ Biosafety  Res. January-June; 6(1-2):15-35. Epub 2007 Oct 26

Monier JM, Bernillon D, Kay E, Faugier A, Rybalka O, Dessaux Y,  Simonet P and TM Vogel. 2007. Detection of potential transgenic plant  DNA recipients among soil bacteria. Environ Biosafety Res.  January-June; 6(1-2):71-83. Epub 2007 Oct 26

Kay, E., Chabrillat, G., Vogel, T.M., Simonet, P. 2003. Intergeneric transfer of chromosomal and conjugative plasmid genes between Ralstonia solanacearum and Acinetobacter sp. BD413. Molecular Plant-Microbe Interactions, 16:74-82.

Ceccherini, MT., Poté, J., Kay, E., Tran Van, V., Maréchal, J., Pietramellara, G., Nannipieri, P., Vogel, T.M., and Simonet, P. 2003. Degradation and transformability of DNA from transgenic plants. Appl. Environ. Microbiol. 69:673-678.

Poté, J. Ceccherini, MT., Van Tran, V., Rosselli, W., Wildi, W., Simonet, P., and Vogel, T.M. 2003. Fate and transport of antibiotic resistance genes in saturated soil columns. European Journal of Soil Biology, 39:65-72.

Kay E., Bertolla F. Vogel T.M and Simonet P. 2002. Opportunistic colonization of Ralstonia solanacearum-infected plants by Acinetobacter sp. and its natural competence development. Microbiol.Ecol. 43:291-297.

Kay E., Vogel T. M., Bertolla F., Nalin R. and Simonet P. 2002. In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria Appl. Environ. Microbiol. 68: 3345-3351.

Bertolla F., Pepin R., Passelegue-Robe E., Paget E., Simkin A., Nesme X. and Simonet P. 2000. Plant Genome Complexity May Be a Factor Limiting In Situ the Transfer of Transgenic Plant Genes to the Phytopathogen Ralstonia solanacearum. Appl. Environ. Microbiol. 66 : 4161-4167

Bertolla F., Kay E. and Simonet P. 2000. Potential dissemination of antibiotic resistance genes from transgenic plants to microorganisms. Infect Control Hosp Epidemiol J. 21 : 390-393

Simonet P. 2000. Evaluation des potentialités de transfert de l'ADN des plantes transgéniques vers les bactéries du sol. OCL 7 : 320-323

Bertolla F., Frostegard A., Brito B., Nesme X. and Simonet P. 1999. During infection of its host, the plant pathogen Ralstonia solanacearum naturally develops a state of competence and exchanges genetic material. Mol. Plant-Microbe Interact., 12, 467-472.

Bertolla F. and Simonet P. 1999. Horizontal gene transfers in the environment : Natural transformation as a putative process for gene transfers between transgenic plants and micro-organisms. Research in Microbiol 150, 5409-5420.