Horizontal Gene Transfer Environmental Microbial Genomics Group

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Laboratoire Ampere
Ecole Centrale de Lyon . France
(33) 472 18 65 14


Horizontal Gene Transfer

The general objectives were to determine the involvement of horizontal gene transfers (HGT) in the adaptation and evolution of bacteria to new environments. Our studies focused mainly on natural genetic transformation and also, but at a lesser extent, on conjugation.



Demanèche S, Monier J-M, Dugat-Bony E and Simonet P. 2011. Exploration of horizontal gene transfer between transplastomic tobacco and plant‐associated bacteria. FEMS Microb. Ecol. 78: 129-136.

Demanèche S, Brard C, Lima O, Binet F and Simonet P. 2011. Development of a new tool to improve gene transfer frequency calculations. J. Microbiol. Methods. 86: 255-257.

Pontiroli A, Rizzi A, Simonet P, Daffonchio D, Vogel T.M. and Monier JM. 2009. Visual evidence of horizontal gene transfer between plant and bacteria in the phytosphere of transplastomic tobacco. Appl. Environ. Microbiol. 75: 3314-3322.

Mercier A, Bertolla F, Passelègue-Robe E and Simonet P. 2009. Influence of DNA conformation and role of comA and recA on natural transformation in Ralstonia solanacearum. Can. J. Microbiol. 55: 762-770.

Demanèche S, Sanguin H, Poté J, Navarro E, Bernillon D, Mavingui P,  Wildi W, Vogel TM and Simonet P. 2008 Antibiotic resistant soil  bacteria in transgenic plant fields. (Proc Natl Acad. Sci. USA) PNAS. 105:3957-3962.

Rizzi A, Pontiroli A, Brusetti L, Borin S, Sorlini C, Abruzzes Ae,  Sacchi GA,   Vogel T. M., Simonet P, Bazzicalupo M, Nielsen KM, Monier  J.M and Daffonchio D. 2008. Strategy for In Situ Detection of Natural  Transformation-Based Horizontal Gene Transfer Events. Appl. Environ.  Microbiol. 74: 1250-1254.

Mercier, A., E. Kay and P. Simonet. 2006. Horizontal gene transfer by natural transformation in soil environment. In Nannipieri, P.and K. Smalla (ed.), Nucleic Acids and Proteins in Soils. Spinger-Verlag, Berlin, Heidelberg.

Cérémonie, H.,Buret, F., Simonet, P., Vogel, T.M. 2006. Natural electrotransformation of lightning-competent Pseudomonas sp. strain N3 in artificial soil microcosms. Appl Environ Microbiol 72: 2385-2389

Ginolhac A., Jarrin C., Robe P., Perrière G., Vogel T. M. , Simonet P. and Nalin R. 2005. Type I PKS may have evolved through horizontal gene transfer. Journal of Molecular Evolution.  60:5.

Kay, E., Chabrillat, G., Vogel, T.M., Simonet, P. 2003. Intergeneric transfer of chromosomal and conjugative plasmid genes between Ralstonia solanacearum and Acinetobacter sp. BD413.  Molecular Plant-Microbe Interactions, 16:74-82.

Kay E., Bertolla F. Vogel T.M and Simonet P. 2002. Opportunistic colonization of Ralstonia solanacearum-infected plants by Acinetobacter sp. and its natural competence development. Microbiol.Ecol.43:291-297.

Demanèche S., Bertolla F., Buret F., Nalin R., Sailland A., Auriol P., Vogel T.M. and Simonet P. 2001. Laboratory-scale evidence for lightning-mediated gene transfer in soil. Appl. Environ. Microbiol. 67: 3440-3444.

Demaneche S., Kay E., Gourbiere F. and Simonet P. 2001. Natural Transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in Soil. Appl. Environ. Microbiol.;67 :2617-2621

Clerc-Bardin S., Karray F., Frostegard A., Pernodet J.-L. and Simonet P. 2001. Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil. FEMS Microbiol. Ecol. 38 : 115-121

Clerc S and Simonet P. 1998. A review of available systems to investigate transfer of DNA to indigenous soil bacteria. Anto. van Leew. 73:15-23.