Microarrays Environmental Microbial Genomics Group

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Laboratoire Ampere
Ecole Centrale de Lyon . France
(33) 472 18 65 14

 
 

Microarrays

Our microarray work involves both phylochip and genomic microarrays in collaboration with regional, national and international partners. We have local production and data acquisition facilities. Financing is provided in part by l'ADEME, Région Rhône-Alpes, et le Ministre de Recherche. A wide range of projects are underway with the general purpose of addressing the following questions:

  • How does human activity effect microbial biodiversity?
  • How does physical separation alter microbial communities and species definitions?
  • How do ecosystems (e.g. plants, bioreactors, human digestive system)control microbial community structure and function?
  • What is the functional biodiversity of microbial communities?
  • What are the intrinsic genetic distances between bacterial communities?

The probes used for the current phylogenetic microarray are found at the university website:

ftp://ftp-adn.ec-lyon.fr/

Publications

Sanguin, H., Remenant, B., Dechesne, A., Thioulouse, J., Vogel, T.M., Nesme, X., Moënne-Loccoz, Y., Grundmann, G.L. 2006. Potential of a 16S rRNA-based taxonomic microarray for analyzing maize rhizosphere effect on Agrobacterium and bacterial communities. Appl. Environ. Microbiol.

Sanguin H., Herrera A., Oger-Desfeux C., Dechesne A., Simonet P., Navarro E., Vogel T.M., Moënne-Loccoz Y., Nesme X., Grundmann G.L. 2005. Development and validation of a prototype 16S rRNA-based taxonomic microarray for Alphaproteobacteria. Environ. Microbiol. 70 :6342-6346.

Souteyrand, Chen, Cloarec, Nesme, Simonet, Navarro and Martin. 2000. Comparison between Electrochemical and Optoelectrochemical Impedance Measurements for Detection of DNA Hybridization. Applied Biochemistry and Biotechnology 89:195-207.

 

   

MICROARRAYS