Dr. Laure Franqueville

CNRS Research Engineer

Laboratoire Ampère, UMR CNRS 5005
Ecole Centrale de Lyon
Université de Lyon
36 avenue Guy de Collongue
69134 Ecully cedex
France







laure.franqueville@ec-lyon.fr
+33 (0) 478 43 37 17

CV (français)

Research Interests

Horizontal Gene Transfer, Recombination, Virology

My current work focuses on bacteriophages of sulphate-reducing bacteria from soils polluted with hydrocarbons. I have also worked on the development of an innovative tool for metagenomics called “Genefish” (a specific strain devoted to gene capture), and the screening of enzymes implicated in the degradation of recalcitrant natural and xenobiotic compounds such as chitinases, laccases, and dehalogenases from metagenomes.

Curriculum Vitae

  • Oct 2008 to present: Environmental Microbial Genomics
    Laboratoire Ampère CNRS UMR 5005/ Ecole Centrale Lyon/ Université de Lyon
  • Sept 2000 – oct 2008: CNRS FRE 3011 (ex UMR 5537) Virologie et Pathologie Humaine                                                                      Directeur Prof. B. LINA. Faculté de Médecine Laennec (Lyon)
  • Déc 1995 – avril 2000 : CNRS/ Aventis UMR1582 Vectorologie et Transfert de gènes                                                                                    Directeur Dr. M. PERRICAUDET. Institut G.Roussy (Villejuif)
  • Oct 1994 – juin 1995 : INSERM CJF93-01 Récepteurs et Signalisation des Interleukines                                                                              (Stage formation permanente) Directeur Dr. J. BERTOGLIO. Faculté de Pharmacie Paris XI
  • Fév 1989 – nov 1993 : SANDOZ PHARMA AG (à ce jour : NOVARTIS, Bâle (SUISSE))                                                                   Département Biotechnologie – Recherche Préclinique

Education

  • Ph.D. Adenovirus-mediated gene transfer (Virology), 2007, Université de Lyon (UCBLyonI), France
  • M.S.Biochemistry (Option: Microbiology), 1987, Université Aix-Marseille I, France
  • CES. Immunology, 1988, Université Aix-Marseille II, France
  • BTS. Biochemistry, 1984, LTE M.Curie Marseille, France

Publications

Microbial soil community analyses for forensic science: Application to a blind test. Demanèche S, Schauser L, Dawson L, Franqueville L, Simonet P. Forensic Sci Int. 2017 Jan;270:153-158. doi: 10.1016/j.forsciint.2016.12.004. Epub 2016 Dec 9.

Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes. Ufarté L, Bozonnet S, Laville E, Cecchini DA, Pizzut-Serin S, Jacquiod S, Demanèche S, Simonet P, Franqueville L, Potocki Veronese G. Methods Mol Biol. 2016;1399:257-71. doi: 10.1007/978-1-4939-3369-3_15.

Linking environmental prokaryotic viruses and their host through CRISPRs. Sanguino L, Franqueville L, Vogel TM, Larose C. FEMS Microbiol Ecol. 2015 May;91(5). pii: fiv046. doi: 10.1093/femsec/fiv046. Epub 2015 Apr 22.

nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip. Menad S, Franqueville L, Haddour N, Buret F, Frenea-Robin M. Acta Biomater. 2015 Apr;17:107-14. doi: 10.1016/j.actbio.2015.01.011. Epub 2015 Jan 14.

Jacquiod S, Demanèche S, Delmont TO, Ausec L, Dunon V, Franqueville L, Mandic-Mulec I, Springael D, Vogel TM and Simonet P. 2014. Genetic screening of a fosmid metagenomic soil library using radio-labeled oligonucleotide probes. J. Biotechnol. (in press).  doi: 10.1016/j.jbiotec.2014.03.036.

Jacquiod S, Franqueville L, Cécillon S, M. Vogel T, Simonet P (2013) Soil Bacterial Community Shifts after Chitin Enrichment: An Integrative Metagenomic Approach. PLoS ONE 8(11): e79699. doi:10.1371/journal.pone.0079699

Soil Metagenomic Exploration of the Rare Biosphere Delmont T, Franqueville L, Jacquiod S, Simonet P, Vogel T. Frans J. de Bruijn. Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches, Wiley-Blackwell, pp.287-298, 2011, 978-0-470-92418-1

Franqueville L, Henning P, Magnusson M, Vigne E, Schoehn G, Blair-Zajdel ME, Habib N, Lindholm L, Blair GE, Hong SS, Boulanger P. 2008. Protein crystals in Adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis. PLoS One. 2008 Aug 6;3(8):e2894.

Waszak P, Franqueville L, Franco-Motoya ML, Rosa-Calatrava M, Boucherat O, Lindholm L, Delacourt C, Boulanger P. 2007. Toxicity of fiber- and penton base-modified adenovirus type 5 vectors on lung development in newborn rats. Mol Ther. 2007 Nov;15(11):2008-16. Epub 2007 Jul 24.

Adenovirus type 5 fiber knob domain has a critical role in fiber protein synthesis and encapsidation. Henning P, Lundgren E, Carlsson M, Frykholm K, Johannisson J, Magnusson MK, Tång E, Franqueville L, Hong SS, Lindholm L, Boulanger P.J Gen Virol. 2006 Nov;87(Pt 11):3151-60.

Enhancement of adenovirus-mediated gene delivery to rheumatoid arthritis synoviocytes and synovium by fiber modifications: role of arginine-glycine-aspartic acid (RGD)- and non-RGD-binding integrins. Toh ML, Hong SS, van de Loo F, Franqueville L, Lindholm L, van den Berg W, Boulanger P, Miossec P.J Immunol. 2005 Dec 1;175(11):7687-98.

The 100K-chaperone protein from adenovirus serotype 2 (Subgroup C) assists in trimerization and nuclear localization of hexons from subgroups C and B adenoviruses. Hong SS, Szolajska E, Schoehn G, Franqueville L, Myhre S, Lindholm L, Ruigrok RW, Boulanger P, Chroboczek J.J Mol Biol. 2005 Sep 9;352(1):125-38.

Gene transduction and cell entry pathway of fiber-modified adenovirus type 5 vectors carrying novel endocytic peptide ligands selected on human tracheal glandular cells. Gaden F, Franqueville L, Magnusson MK, Hong SS, Merten MD, Lindholm L, Boulanger P. J Virol. 2004 Jul;78(13):7227-47.

Mitogenic activity of Epstein-Barr virus-encoded BARF1 protein. Sall A, Caserta S, Jolicoeur P, Franqueville L, de Turenne-Tessier M, Ooka T. Oncogene. 2004 Jun 17;23(28):4938-44.

Integrin alpha3beta1 is an alternative cellular receptor for adenovirus serotype 5. Salone B, Martina Y, Piersanti S, Cundari E, Cherubini G, Franqueville L, Failla CM, Boulanger P, Saggio I. J Virol. 2003 Dec;77(24):13448-54.

Identification of adenovirus (ad) penton base neutralizing epitopes by use of sera from patients who had received conditionally replicative ad (addl1520) for treatment of liver tumors. Hong SS, Habib NA, Franqueville L, Jensen S, Boulanger PA. J Virol. 2003 Oct;77(19):10366-75.

Mechanism of restriction of normal and cystic fibrosis transmembrane conductance regulator-deficient human tracheal gland cells to adenovirus infection and ad-mediated gene transfer. Gaden F, Franqueville L, Hong SS, Legrand V, Figarella C, Boulanger P. Am J Respir Cell Mol Biol. 2002 Nov;27(5):628-40.